Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel

Cell. 1987 Jul 31;50(3):405-13. doi: 10.1016/0092-8674(87)90494-6.

Abstract

The Drosophila Shaker (Sh) gene appears to encode a type of voltage-sensitive potassium (K+) channel called the A channel. We have isolated Sh as part of a 350 kb chromosomal walk. The region around Sh contains four identified transcription units. We find that Sh corresponds to a very large transcription unit encompassing a total of about 95 kb of genomic DNA and split by a major 85 kb intron. Sh has multiple hydrophobic domains that have a high probability of being membrane-spanning, consistent with the proposal that it encodes an ion channel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA Restriction Enzymes / metabolism
  • Drosophila / genetics*
  • Gene Expression Regulation
  • Genes*
  • Ion Channels*
  • Translocation, Genetic

Substances

  • Ion Channels
  • DNA Restriction Enzymes

Associated data

  • GENBANK/M17155