Phenocopies induced with antisense RNA identify the wingless gene

Cell. 1987 Aug 14;50(4):659-63. doi: 10.1016/0092-8674(87)90039-0.


Earlier work suggested that the wingless gene of Drosophila is required for cooperation within discrete groups of cells during development. We show that antisense RNA made from a 3 kb transcript produces wingless mutant phenocopies when injected into wild-type eggs, proving that this transcript executes the wingless function. In the accompanying paper, Rijsewijk et al. report cloning of a Drosophila homolog (Dint-1) of the mouse int-1 gene and show that this gene is identical to wingless+. The partial sequence of the wingless cDNA that we have isolated is identical to part of the complete Dint-1 sequence described by Rijsewijk et al.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA / genetics
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Genes*
  • Insect Hormones / genetics*
  • Larva / ultrastructure
  • Mice / genetics
  • Morphogenesis
  • Phenotype
  • RNA / genetics*
  • RNA, Antisense
  • Sequence Homology, Nucleic Acid


  • Insect Hormones
  • RNA, Antisense
  • RNA
  • DNA