Enhanced flowType/RchyOptimyx: a BioConductor pipeline for discovery in high-dimensional cytometry data

Bioinformatics. 2014 May 1;30(9):1329-30. doi: 10.1093/bioinformatics/btt770. Epub 2014 Jan 8.


We present a significantly improved version of the flowType and RchyOptimyx BioConductor-based pipeline that is both 14 times faster and can accommodate multiple levels of biomarker expression for up to 96 markers. With these improvements, the pipeline is positioned to be an integral part of data analysis for high-throughput experiments on high-dimensional single-cell assay platforms, including flow cytometry, mass cytometry and single-cell RT-qPCR.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Biomarkers / analysis
  • Flow Cytometry / methods*
  • Software


  • Antigens, CD
  • Biomarkers