Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation

Photochem Photobiol Sci. 2014 Mar;13(3):531-40. doi: 10.1039/c3pp50351h. Epub 2014 Jan 9.


Grp1-associated scaffold protein (Grasp), the product of a retinoic acid-induced gene in P19 embryonal carcinoma cells, is expressed primarily in brain, heart, and lung of the mouse. We report herein that Grasp transcripts are also found in mouse skin in which the Grasp gene is robustly induced following acute ultraviolet-B (UVB) exposure. Grasp(-/-) mice were found to exhibit delayed epidermal proliferation and a blunted apoptotic response after acute UVB exposure. Immunohistochemical analyses revealed that the nuclear residence time of the tumor suppressor protein p53 was reduced in Grasp(-/-) mice after UVB exposure. Taken together, our results suggest that a physiological role of Grasp may be to regulate skin homeostasis after UVB exposure, potentially by influencing p53-mediated apoptotic responses in skin.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Apoptosis / radiation effects
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Nucleus / physiology
  • Cell Nucleus / radiation effects
  • Cell Proliferation / radiation effects
  • Cells, Cultured
  • Dermis / physiology
  • Dermis / radiation effects
  • Epidermis / pathology
  • Epidermis / physiology
  • Epidermis / radiation effects
  • Fibroblasts / physiology
  • Fibroblasts / radiation effects
  • Homeostasis / physiology
  • Homeostasis / radiation effects
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Skin / radiation effects*
  • Skin Physiological Phenomena / radiation effects*
  • Tumor Suppressor Protein p53 / metabolism
  • Ultraviolet Rays


  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Tumor Suppressor Protein p53
  • tamalin protein, mouse