Sites of Action of Two Ribosomal RNA Methylases Responsible for Resistance to Aminoglycosides

J Mol Biol. 1987 Feb 20;193(4):661-71. doi: 10.1016/0022-2836(87)90349-4.

Abstract

Methylation of either of two residues (G-1405 or A-1408) within bacterial 16 S ribosomal RNA results in high level resistance to specific combinations of aminoglycoside antibiotics. The product of a gene that originated in Micromonospora purpurea (an actinomycete that produces gentamicin) gives resistance to kanamycin plus gentamicin by converting residue G-1405 to 7-methylguanosine. Resistance to kanamycin plus apramycin results from conversion of residue A-1408 to 1-methyladenosine catalysed by the product of a gene from Streptomyces tenjimariensis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / pharmacology*
  • Base Sequence
  • Binding Sites*
  • Drug Resistance, Microbial
  • Escherichia coli / enzymology
  • Methylation
  • Methyltransferases / metabolism*
  • Nucleic Acid Hybridization
  • RNA
  • RNA, Bacterial / metabolism
  • RNA, Ribosomal / metabolism
  • Streptomyces / enzymology

Substances

  • Aminoglycosides
  • RNA primers
  • RNA, Bacterial
  • RNA, Ribosomal
  • RNA
  • Methyltransferases
  • rRNA (adenosine-O-2'-)methyltransferase