Nanoscopy of filamentous actin in cortical dendrites of a living mouse

Biophys J. 2014 Jan 7;106(1):L01-3. doi: 10.1016/j.bpj.2013.11.1119.


We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 μm in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Actin Cytoskeleton / ultrastructure*
  • Animals
  • Dendrites / metabolism
  • Dendrites / ultrastructure*
  • Mice
  • Microscopy, Fluorescence / methods*
  • Visual Cortex / metabolism
  • Visual Cortex / ultrastructure*