Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue

Nat Methods. 2014 Feb;11(2):190-6. doi: 10.1038/nmeth.2804. Epub 2014 Jan 12.


Transcriptome profiling of single cells resident in their natural microenvironment depends upon RNA capture methods that are both noninvasive and spatially precise. We engineered a transcriptome in vivo analysis (TIVA) tag, which upon photoactivation enables mRNA capture from single cells in live tissue. Using the TIVA tag in combination with RNA sequencing (RNA-seq), we analyzed transcriptome variance among single neurons in culture and in mouse and human tissue in vivo. Our data showed that the tissue microenvironment shapes the transcriptomic landscape of individual cells. The TIVA methodology is, to our knowledge, the first noninvasive approach for capturing mRNA from live single cells in their natural microenvironment.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain / metabolism*
  • Computational Biology
  • Gene Expression Profiling*
  • Gene Library
  • High-Throughput Nucleotide Sequencing / methods*
  • Hippocampus / metabolism*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Neurons / metabolism*
  • RNA, Messenger / genetics
  • Sequence Analysis, RNA / methods*


  • RNA, Messenger

Associated data

  • GEO/GSE52525