Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells

Sci Rep. 2014 Jan 13;4:3652. doi: 10.1038/srep03652.

Abstract

Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase / genetics*
  • ADP-ribosyl Cyclase / metabolism
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • Cell Differentiation / genetics*
  • Cell Movement / genetics
  • Down-Regulation
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism
  • GPI-Linked Proteins / genetics
  • GPI-Linked Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Humans
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / metabolism*
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Osteogenesis / genetics*
  • Phosphatidylinositol 3-Kinases / metabolism
  • Protein Binding
  • Protein Biosynthesis
  • Signal Transduction

Substances

  • Antigens, CD
  • GPI-Linked Proteins
  • Nerve Tissue Proteins
  • SCRG1 protein, human
  • Phosphatidylinositol 3-Kinases
  • Focal Adhesion Protein-Tyrosine Kinases
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases
  • ADP-ribosyl Cyclase
  • ADP-ribosyl cyclase 2