Improved method for the isolation of RNA from plant tissues

Anal Biochem. 1987 May 15;163(1):16-20. doi: 10.1016/0003-2697(87)90086-8.

Abstract

A fast and efficient method for the isolation of RNA from plant tissues is described. Tuber tissue is homogenized in a guanidine hydrochloride-containing buffer followed by direct extraction with phenol/chloroform. The RNA is precipitated from the aqueous phase, washed with 3 M sodium acetate and 70% ethanol, and finally dissolved in water. The yield of RNA is up to 500 micrograms/g of tissue and several tests indicate intact and nondegraded RNA. This method can be adapted to a small-scale version by the use of 1.5-ml tubes, allowing rapid isolation of RNA from a larger number of samples. Finally, this method is of particular use for isolating RNA from tissues with a high polysaccharide and nuclease content such as wounded potato tubers.

MeSH terms

  • Chloroform
  • Electrophoresis, Agar Gel
  • Ethanol
  • Guanidine
  • Guanidines
  • Nucleic Acid Hybridization
  • Pentanols
  • Phenol
  • Phenols
  • Plants, Toxic*
  • RNA / isolation & purification*
  • Solanum tuberosum / genetics*
  • Tobacco / genetics*

Substances

  • Guanidines
  • Pentanols
  • Phenols
  • Phenol
  • Ethanol
  • RNA
  • Chloroform
  • isopentyl alcohol
  • Guanidine