Matrix metalloproteinase 3 promotes cellular anti-dengue virus response via interaction with transcription factor NFκB in cell nucleus

PLoS One. 2014 Jan 8;9(1):e84748. doi: 10.1371/journal.pone.0084748. eCollection 2014.


Dengue virus (DENV), the causative agent of human Dengue hemorrhagic fever, is a mosquito-borne virus of immense global health importance. Characterization of cellular factors promoting or inhibiting DENV infection is important for understanding the mechanism of DENV infection. In this report, MMP3 (stromelysin-1), a secretory endopeptidase that degrades extracellular matrices, has been shown promoting cellular antiviral response against DENV infection. Quantitative RT-PCR and Western Blot showed that the expression of MMP3 was upregulated in DENV-infected RAW264.7 cells. The intracellular viral loads were significantly higher in MMP3 silenced cells compared with controls. The expression level of selective anti-viral cytokines were decreased in MMP3 siRNA treated cells, and the transcription factor activity of NFκB was significantly impaired upon MMP3 silencing during DENV infection. Further, we found that MMP3 moved to cell nucleus upon DENV infection and colocalized with NFκB P65 in nucleus. Co-immunoprecipitation analysis suggested that MMP3 directly interacted with NFκB in nucleus during DENV infection and the C-terminal hemopexin-like domain of MMP3 was required for the interaction. This study suggested a novel role of MMP3 in nucleus during viral infection and provided new evidence for MMPs in immunomodulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Animals
  • Cell Line
  • Cell Nucleus / metabolism*
  • Cytokines / genetics
  • Cytoplasm / metabolism
  • Dengue Virus / physiology*
  • Down-Regulation
  • Gene Silencing
  • Humans
  • Matrix Metalloproteinase 3 / chemistry
  • Matrix Metalloproteinase 3 / deficiency
  • Matrix Metalloproteinase 3 / genetics
  • Matrix Metalloproteinase 3 / metabolism*
  • Mice
  • NF-kappa B / metabolism*
  • Protein Binding
  • Protein Structure, Tertiary
  • RNA, Small Interfering / genetics
  • Up-Regulation


  • Cytokines
  • NF-kappa B
  • RNA, Small Interfering
  • Matrix Metalloproteinase 3

Grant support

This work was supported by a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) (IRT1075), National Natural Science Foundation of China (NSFC) (81172812, 81271792, 31200648) and Jiangsu Natural Science Foundation (BK2012180), J.D. is a member of Jiangsu Provincial Innovative Research Team. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.