Induction of angiogenesis by mixtures of two angiogenic proteins, angiogenin and acidic fibroblast growth factor, in the chick chorioallantoic membrane

Biochem Biophys Res Commun. 1987 Aug 14;146(3):1122-31. doi: 10.1016/0006-291x(87)90764-9.


The chick chorioallantoic membrane assay was employed to assess the angiogenic response induced by mixtures of human angiogenin with bovine heparin-binding acidic fibroblast growth factor. Statistical evaluation of data accumulated at several molar ratios of the two proteins indicate that the angiogenic activity observed is neither an additive nor a synergistic resultant of the activities of the proteins separately. The possibility exists, however, that at an approximately 1:1 mole ratio an apparent inhibitory effect can be observed. Mechanisms which could underlie such observed effects are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allantois / blood supply*
  • Allantois / drug effects
  • Angiogenesis Inducing Agents / blood
  • Angiogenesis Inducing Agents / isolation & purification
  • Angiogenesis Inducing Agents / pharmacology*
  • Animals
  • Cattle
  • Chick Embryo
  • Chorion / blood supply*
  • Chorion / drug effects
  • Extraembryonic Membranes / blood supply*
  • Fibroblast Growth Factors / pharmacology*
  • Growth Substances / pharmacology*
  • Humans
  • Neovascularization, Pathologic*


  • Angiogenesis Inducing Agents
  • Growth Substances
  • Fibroblast Growth Factors