Aerococcus vaginalis sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of Aerococcus suis, Aerococcus viridans, Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola

Int J Syst Evol Microbiol. 2014 Apr;64(Pt 4):1229-1236. doi: 10.1099/ijs.0.058081-0. Epub 2014 Jan 13.

Abstract

A gram-stain-positive, facultatively anaerobic, non-spore-forming, catalase-negative, coccoid-shaped bacterial strain, designated BV2(T), was isolated from the vaginal mucosa of a beef cow in Japan. Phylogenetic analysis showed that the isolate shared high 16S rRNA gene sequence similarity (92.9 %) with Aerococcus suis 1821/02(T) and low similarity (<92.7 %) with any other recognized species of the genus Aerococcus. The DNA G+C content was 44.7 mol%, which is within the range observed among species of the genus Aerococcus (37.5-48.4 mol%). The major cellular fatty acid was C18 : 1ω9c, similar to other type strains of species of the genus Aerococcus. The results of genotypic, phenotypic and chemotaxonomic analyses as well as the low degree of DNA-DNA relatedness with all recognized members of the genus Aerococcus indicate that strain BV2(T) represents a novel species of the genus Aerococcus, for which the name Aerococcus vaginalis sp. nov. is proposed. The type strain is BV2(T) ( = JCM 19163(T) = DSM 27293(T)). Emended descriptions of Aerococcus suis, Aerococcus viridans, Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola are also presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerococcus / classification*
  • Aerococcus / genetics
  • Aerococcus / isolation & purification
  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • Cattle / microbiology*
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Female
  • Japan
  • Molecular Sequence Data
  • Mucous Membrane / microbiology
  • Nucleic Acid Hybridization
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Vagina / microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AB818493