The copper (II) ion as a carrier for the antibiotic capreomycin against Mycobacterium tuberculosis

Bioorg Med Chem Lett. 2014 Feb 1;24(3):976-82. doi: 10.1016/j.bmcl.2013.12.053. Epub 2013 Dec 28.

Abstract

In recent years, the bacterium responsible for tuberculosis has been increasing its resistance to antibiotics resulting in new multidrug resistant Mycobacterium tuberculosis (MR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study we use several analytical techniques including NMR, FT-ICR, TOF-MS, LC-MS and UV/Vis to study the copper-capreomycin complex. The copper (II) cation is used as a carrier for the antibiotic capreomycin. Once this structure was studied using NMR, FT-ICR, and MALDI-TOF-MS, the NIH-NIAID tuberculosis cell line for several Tb strains (including antibiotic resistant strains) were tested against up to seven variations of the copper-capreomycin complex. Different variations of copper improved the efficacy of capreomycin against Tb up to 250 fold against drug resistant strains of Tb.

Keywords: Antibiotic; Capreomycin; Drug delivery; FT-ICR; Mass spec; Mycobacterium tuberculosis; NMR; Tb.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibiotics, Antitubercular / chemistry
  • Antibiotics, Antitubercular / pharmacology
  • Capreomycin / chemistry*
  • Capreomycin / pharmacology*
  • Copper* / chemistry
  • Drug Resistance, Bacterial / drug effects*
  • Ions
  • Molecular Structure
  • Mycobacterium tuberculosis / drug effects*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Antibiotics, Antitubercular
  • Ions
  • Capreomycin
  • Copper