Real-time monitoring of the accretion of Rhizopus oligosporus biomass during the solid-substrate tempe fermentation

World J Microbiol Biotechnol. 1991 Mar;7(2):248-59. doi: 10.1007/BF00328998.


We describe a novel method for the real-time estimation of the accretion of blomass during the solid-substrate tempe fermentation of soy beans, lupins and quinoa by Rhizopus oligosporus Salto. The method is based on measurements of the dielectric permittivity at radio-frequencies, using a four-terminal instrument (the Bugmeter). In all cases, excellent Ilnearity is observed during the growth phase between the dielectric permittivity and the hyphal length as determined microscopically.