Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia

Int J Syst Evol Microbiol. 2014 Mar;64(Pt 3):963-967. doi: 10.1099/ijs.0.054015-0. Epub 2013 Dec 18.

Abstract

A taxonomic study was carried out on strain GCS-AN-3(T), which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3(T) were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3(T) was most closely related to Ottowia pentelensis DSM 21699(T) (96.2 %). The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and cyclo C17 : 0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3(T) is considered to represent a novel species of the genus Ottowia, for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3(T) = LMG 27179(T) = CGMCC 1.12324(T) = MCCC 1A01410(T)). An emended description of the genus Ottowia is also proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • China
  • Coke / microbiology*
  • Comamonadaceae / classification*
  • Comamonadaceae / genetics
  • Comamonadaceae / isolation & purification
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Molecular Sequence Data
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sewage / microbiology*
  • Ubiquinone / chemistry
  • Waste Water / microbiology

Substances

  • Coke
  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Sewage
  • Waste Water
  • Ubiquinone
  • ubiquinone 8

Associated data

  • GENBANK/KC755038