Pacific white shrimp, Litopenaeus vannamei has been introduced recently for culture practice in India. Though SPF stocks are imported for larval production and thereafter culture practice, these are prone to infection with the existing viruses in the environment. Here we report mortality of L.vannamei in several farms in India with minimum biosecurity. The shrimp were harvested early within 50-72 days of culture due to the onset of disease and consequent mortality. As per the analysis carried out, the shrimp were infected with two virus, white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV). About 80 % of the samples collected had either or both of the viruses. A majority of these samples (60 %) had dual infection with WSSV and IHHNV. Infection of shrimp with WSSV and IHHNV could be detected both by PCR and histopathology. Some of the samples had either exclusively WSSV infection or IHHNV infection and were also harvested before the completion of the required culture period. All the samples analyzed were negative for taura syndrome virus, yellow head virus and infectious myonecrosis virus. While it is difficult to point out the exact etiological agent as the cause of mortality, strict biosecurity measures are advisable for the continuity of L. vannamei culture in India.
Keywords: Dual viral infection; India; Litopenaeus vannamei; Shrimp mortality.
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White-spot syndrome virus (WSSV) introduction into the Gulf of Mexico and Texas freshwater systems through imported, frozen bait-shrimp.Dis Aquat Organ. 2006 Jul 25;71(2):91-100. doi: 10.3354/dao071091. Dis Aquat Organ. 2006. PMID: 16956056
Competition of infectious hypodermal and haematopoietic necrosis virus (IHHNV) with white spot syndrome virus (WSSV) for binding to shrimp cellular membrane.J Fish Dis. 2016 Oct;39(10):1225-9. doi: 10.1111/jfd.12489. Epub 2016 May 12. J Fish Dis. 2016. PMID: 27169386
Historic emergence, impact and current status of shrimp pathogens in Asia.J Invertebr Pathol. 2012 Jun;110(2):166-73. doi: 10.1016/j.jip.2012.03.004. Epub 2012 Mar 10. J Invertebr Pathol. 2012. PMID: 22429834 Review.
Immunological-based assays for specific detection of shrimp viruses.World J Virol. 2014 Feb 12;3(1):1-10. doi: 10.5501/wjv.v3.i1.1. World J Virol. 2014. PMID: 24567913 Free PMC article. Review.
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