Dynamic assembly/disassembly processes of photoresponsive DNA origami nanostructures directly visualized on a lipid membrane surface

J Am Chem Soc. 2014 Feb 5;136(5):1714-7. doi: 10.1021/ja4109819. Epub 2014 Jan 24.


Here, we report the direct visualization of the assembly/disassembly processes of photoresponsive DNA origami nanostructures which can be placed on a lipid bilayer surface. The observation relies on controlled interactions between the bilayer components and cholesterol moieties introduced to the hexagonal origami structures, one of whose outer edges carries Azo-ODNs. The bilayer-placed hexagonal dimer was disassembled into monomer units by UV irradiation, and reversibly assembled again during visible light irradiation. These dynamic processes were directly monitored with high-speed atomic force microscopy. The successful application of our approach should facilitate studies of interactive and functional behaviors of various DNA nanostructures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholesterol / chemistry
  • DNA / chemistry*
  • DNA / ultrastructure
  • Lipid Bilayers / chemistry*
  • Membranes, Artificial*
  • Microscopy, Atomic Force
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Nucleic Acid Conformation*
  • Photochemical Processes
  • Sphingomyelins / chemistry*


  • Lipid Bilayers
  • Membranes, Artificial
  • Sphingomyelins
  • DNA
  • Cholesterol