Anti-inflammatory and antiapoptotic effect of interleukine-18 binding protein on the spinal cord ischemia-reperfusion injury

Inflammation. 2014 Jun;37(3):917-23. doi: 10.1007/s10753-014-9811-7.

Abstract

We investigated the anti-inflammatory and antiapoptotic effects of interleukin-18 binding protein (IL-18BP) on spinal cord ischemia/reperfusion (I/R) injury in rats. Twenty-one adult male rats were divided into three groups: sham, I/R, and I/R+IL-18BP. Proinflammatory cytokines were determined in rat blood samples by using ELISA, while apoptosis was immunohistochemically evaluated in spinal cord tissue using caspase 3. Both IL-18 and TNF-α were significantly decreased in the IL-18BP group compared to that in the sham group. The highest caspase 3 levels were observed in the I/R group, while the lowest levels were found in the sham group. The mean Tarlov score of the I/R group was significantly lower than that of the sham group. However, the mean Tarlov score of the IL-18BP group was significantly higher than that of the I/R group. The results of the current study demonstrate that IL-18BP plays both anti-inflammatory and antiapoptotic roles in spinal cord I/R injury.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Apoptosis / drug effects
  • Apoptosis Regulatory Proteins / pharmacology*
  • Caspase 3 / metabolism
  • Cytokines / blood
  • Disease Models, Animal
  • Intercellular Signaling Peptides and Proteins / pharmacology*
  • Interleukin-18 / blood
  • Male
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / drug therapy*
  • Spinal Cord
  • Spinal Cord Ischemia / drug therapy*
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Anti-Inflammatory Agents
  • Apoptosis Regulatory Proteins
  • Cytokines
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-18
  • Tumor Necrosis Factor-alpha
  • interleukin-18 binding protein
  • Caspase 3