Class I MHC molecules rather than other mouse genes dictate influenza epitope recognition by cytotoxic T cells

Immunogenetics. 1987;26(4-5):267-72. doi: 10.1007/BF00346521.

Abstract

Influenza nucleoprotein (NP) is an important target antigen for influenza A virus cross-reactive cytotoxic T cells (Tc). Here we examine the NP epitope recognized by cloned and polyclonal BALB/c Tc and the genetics of this recognition pattern. We can define NP residues 147-161 as the epitope seen in conjunction with Kd, the only H-2d class I responder allele for NP restriction. H-2d/H-2b F1 mice (C57BL X DBA/2) primed by influenza infection lyse only H-2d target cells treated with peptide 147-161 while H-2b targets are recognized only after treatment with NP residues 365-379 (previously found to be recognized by Db restricted Tc cells). Tc cell recognition of NP peptide 147-161 is entirely dictated by expression of Kd and not by other B10 or C3H background genes of congenic mice. Restriction of a unique NP sequence by each responder class I major histocompatibility complex (MHC) allele suggests that antigen and class I MHC interact for Tc recognition.

MeSH terms

  • Animals
  • Cell Line
  • Cross Reactions
  • Epitopes / analysis*
  • Histocompatibility Antigens / genetics*
  • Influenza A virus / immunology*
  • Major Histocompatibility Complex*
  • Mice
  • Mice, Inbred BALB C
  • Nucleocapsid Proteins
  • Nucleoproteins / immunology*
  • RNA-Binding Proteins*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Core Proteins*
  • Viral Proteins / immunology*

Substances

  • Epitopes
  • Histocompatibility Antigens
  • NP protein, Influenza A virus
  • Nucleocapsid Proteins
  • Nucleoproteins
  • RNA-Binding Proteins
  • Viral Core Proteins
  • Viral Proteins