Combinatorial biosynthesis of sapogenins and saponins in Saccharomyces cerevisiae using a C-16α hydroxylase from Bupleurum falcatum

Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1634-9. doi: 10.1073/pnas.1323369111. Epub 2014 Jan 13.

Abstract

The saikosaponins comprise oleanane- and ursane-type triterpene saponins that are abundantly present in the roots of the genus Bupleurum widely used in Asian traditional medicine. Here we identified a gene, designated CYP716Y1, encoding a cytochrome P450 monooxygenase from Bupleurum falcatum that catalyzes the C-16α hydroxylation of oleanane- and ursane-type triterpenes. Exploiting this hitherto unavailable enzymatic activity, we launched a combinatorial synthetic biology program in which we combined CYP716Y1 with oxidosqualene cyclase, P450, and glycosyltransferase genes available from other plant species and reconstituted the synthesis of monoglycosylated saponins in yeast. Additionally, we established a culturing strategy in which applying methylated β-cyclodextrin to the culture medium allows the sequestration of heterologous nonvolatile hydrophobic terpenes, such as triterpene sapogenins, from engineered yeast cells into the growth medium, thereby greatly enhancing productivity. Together, our findings provide a sound base for the development of a synthetic biology platform for the production of bioactive triterpene sapo(ge)nins.

Keywords: amyrin; cyclodextrin; metabolic engineering; triterpenoid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bupleurum / enzymology*
  • Combinatorial Chemistry Techniques*
  • Culture Media
  • Hydroxylation
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Sapogenins / metabolism*
  • Saponins / biosynthesis*
  • Steroid 16-alpha-Hydroxylase / genetics*

Substances

  • Culture Media
  • RNA, Messenger
  • Sapogenins
  • Saponins
  • Steroid 16-alpha-Hydroxylase

Associated data

  • GENBANK/KC963423