The complete mitochondrial genome of Microhyl pulchra was determined in this work. This mitogenome was 16,744 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (CR). The following four distinctive features were observed: a protein-coding gene (ND1) began with GTG as start codon; eight protein-coding genes (ND1, COII, ATP6, COIII, ND3, ND4, ND5 and Cytb) ended with incomplete stop codon T; four tRNA genes positions (tRNA-Leu (CUN)/tRNA-Thr/Trna-Pro/tRNA-Phe) located between CR and 12S rRNA genes, which was a novel mtDNA gene rearrangement in amphibians; there was no significant repeat regions in the CR.
Keywords: Gene rearrangement; Microhyla pulchra; mitochondrial genome.