Efficacy of combined jejunal and colonic fecal microbiota transplantation for recurrent Clostridium difficile Infection

Clin Gastroenterol Hepatol. 2014 Sep;12(9):1572-6. doi: 10.1016/j.cgh.2013.12.032. Epub 2014 Jan 16.

Abstract

The prevalence of recurrent Clostridium difficile infection (RCDI) is increasing; fecal microbiota transplantation (FMT) is an effective therapy. However, there have been no studies of the efficacy of a single session of combined enteral and colonic FMT or characterizations of changes in the microbiota between donors and recipients. We performed a study of 27 patients with RCDI who were given a fixed volume of processed fecal filtrate via enteroscopy and colonoscopy in a single session. Patients were closely monitored, and fecal samples were collected from 2 patient-donor pairs for 16S rRNA analysis. All patients had reduced stool frequency, abdominal pain, white blood cell counts, and elimination of fecal C difficile toxin (P < .05). FMT increased microbial diversity, increasing proportions of Lachnospiraceae (phylum Firmicutes) and reducing proportions of Enterobacteriaceae. FMT was associated with marked changes in the composition of fecal microbiota in 2 patients with RCDI.

Keywords: 16s rRNA Analysis; Clostridium difficile; Colonic; FMT; Fecal Microbiota Transplantation; Jejunal; Lachnospiraceae; Microbiota; RCDI; Recurrent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biodiversity
  • Biological Therapy / methods*
  • Biota
  • Clostridium Infections / therapy*
  • Diarrhea / therapy*
  • Feces / microbiology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Recurrence
  • Sequence Analysis, DNA
  • Treatment Outcome
  • Young Adult

Associated data

  • SRA/SRA054908