A simple method for the determination of reduction potentials in heme proteins

FEBS Lett. 2014 Mar 3;588(5):701-4. doi: 10.1016/j.febslet.2013.12.030. Epub 2014 Jan 17.

Abstract

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (Em=-115 mV ± 2 mV, pH 7.0) and human CLOCK (Em=-111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

Keywords: Heme; Heme protein; Redox; Reduction potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic Helix-Loop-Helix Transcription Factors / chemistry*
  • CLOCK Proteins / chemistry*
  • Fluorescent Dyes / chemistry
  • Heme / chemistry*
  • Hemeproteins / chemistry*
  • Humans
  • Nerve Tissue Proteins / chemistry*
  • Oxazines / chemistry
  • Oxidation-Reduction
  • Spectrum Analysis

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Fluorescent Dyes
  • Hemeproteins
  • NPAS2 protein, human
  • Nerve Tissue Proteins
  • Oxazines
  • Nile Blue
  • Heme
  • CLOCK Proteins
  • CLOCK protein, human