Regulation of chandelier cell cartridge and bouton development via DOCK7-mediated ErbB4 activation

Cell Rep. 2014 Jan 30;6(2):254-63. doi: 10.1016/j.celrep.2013.12.034. Epub 2014 Jan 16.


Chandelier cells (ChCs), typified by their unique axonal morphology, are the most distinct interneurons present in cortical circuits. Via their distinctive axonal terminals, called cartridges, these cells selectively target the axon initial segment of pyramidal cells and control action potential initiation; however, the mechanisms that govern the characteristic ChC axonal structure have remained elusive. Here, by employing an in utero electroporation-based method that enables genetic labeling and manipulation of ChCs in vivo, we identify DOCK7, a member of the DOCK180 family, as a molecule essential for ChC cartridge and bouton development. Furthermore, we present evidence that DOCK7 functions as a cytoplasmic activator of the schizophrenia-associated ErbB4 receptor tyrosine kinase and that DOCK7 modulates ErbB4 activity to control ChC cartridge and bouton development. Thus, our findings define DOCK7 and ErbB4 as key components of a pathway that controls the morphological differentiation of ChCs, with implications for the pathogenesis of schizophrenia.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cerebral Cortex / cytology
  • Cerebral Cortex / growth & development*
  • Cerebral Cortex / metabolism
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism
  • GTPase-Activating Proteins
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism*
  • Interneurons / metabolism*
  • Mice
  • Presynaptic Terminals / metabolism*
  • Receptor, ErbB-4


  • Dock7 protein, mouse
  • GTPase-Activating Proteins
  • Guanine Nucleotide Exchange Factors
  • ErbB Receptors
  • Erbb4 protein, mouse
  • Receptor, ErbB-4