Antioxidants and tumor necrosis factor alpha-inhibiting activity of sesame oil against doxorubicin-induced cardiotoxicity

Ther Adv Cardiovasc Dis. 2014 Feb;8(1):4-11. doi: 10.1177/1753944713516532. Epub 2014 Jan 17.


Objective: Oxidative stress is currently considered to be the key factor in doxorubicin-induced cardiotoxicity. Comparatively small quantity of the endogenous antioxidant content of the heart is assumed to be the predisposing factor for doxorubicin-induced cardiotoxicity. The present research was designed to evaluate the antioxidant potential and tumor necrosis factor alpha-(TNF-α) inhibiting activity of sesame oil against acute doxorubicin-induced cardiotoxicity.

Methods: Male Wistar albino rats (180-200 g) were administered sesame oil in two dissimilar doses (5 and 10 ml/kg body weight, orally) for 30 days, followed by a single dose of doxorubicin (30 mg/kg s.c.).

Results: In the doxorubicin-treated group, increased oxidative stress was proven by a significant rise of thiobarbituric acid reactive substances level and a decrease of myocardial superoxide dismutase, catalase and reduced glutathione content. Histopathological studies showed myocardial necrosis with accumulation of inflammatory cells, vacuolization and overall enlargement of the myocardium. Western blot analysis showed marked expression of TNF-α in the myocardium. Alteration in biochemical parameters by doxorubicin administration was prevented significantly (p < 0.0001) in the 5 and 10 ml/kg sesame oil treated rat hearts. Treatment with 5 and 10 ml/kg of sesame oil reduced the doxorubicin-induced TNF-α expression in the myocardium, which was associated with reduced myocyte injury. The overall effect of sesame oil was comparable with probucol, which shows similar protection.

Conclusion: The chronic oral administration of sesame oil prevents acute doxorubicin-induced cardiotoxicity by enhancing cardiac endogenous antioxidants and decreasing myocardial TNF-α expression.

Keywords: adriamycin; cardiac myopathy; cardiovascular disease; cytokines; nutritional therapy.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Oral
  • Animals
  • Antibiotics, Antineoplastic / toxicity
  • Antioxidants / metabolism*
  • Dose-Response Relationship, Drug
  • Doxorubicin / toxicity*
  • Heart Diseases / chemically induced
  • Heart Diseases / prevention & control*
  • Male
  • Myocardium / pathology
  • Myocytes, Cardiac / pathology
  • Oxidative Stress / drug effects
  • Probucol / pharmacology
  • Rats
  • Rats, Wistar
  • Sesame Oil / administration & dosage
  • Sesame Oil / pharmacology*
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors


  • Antibiotics, Antineoplastic
  • Antioxidants
  • Tumor Necrosis Factor-alpha
  • Sesame Oil
  • Doxorubicin
  • Probucol