3D single molecule tracking and superresolution microscopy using multifocal plane microscopy

Proc IEEE Int Symp Biomed Imaging. 2012 Dec 31;2012:914-915. doi: 10.1109/ISBI.2012.6235702.


The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.

Keywords: 2D/3D localization accuracy; Cramer-Rao lower bound; Fisher information matrix; maximum likelihood estimator; resolution limits.