The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production

New Phytol. 2014 Apr;202(2):509-20. doi: 10.1111/nph.12676. Epub 2014 Jan 21.

Abstract

Fiber elongation is the key determinant of fiber quality and output in cotton (Gossypium hirsutum). Although expression profiling and functional genomics provide some data, the mechanism of fiber development is still not well understood. Here, a gene encoding a calcium sensor, GhCaM7, was isolated based on its high expression level relative to other GhCaMs in fiber cells at the fast elongation stage. The level of expression of GhCaM7 in the wild-type and the fuzzless/lintless mutant correspond to the presence and absence, respectively, of fiber initials. Overexpressing GhCaM7 promotes early fiber elongation, whereas GhCaM7 suppression by RNAi delays fiber initiation and inhibits fiber elongation. Reactive oxygen species (ROS) play important roles in early fiber development. ROS induced by exogenous hydrogen peroxide (H2 O2 ) and Ca(2+) starvation promotes early fiber elongation. GhCaM7 overexpression fiber cells show increased ROS concentrations compared with the wild-type, while GhCaM7 RNAi fiber cells have reduced concentrations. Furthermore, we show that H2 O2 enhances Ca(2+) influx into the fiber and feedback-regulates the expression of GhCaM7. We conclude that GhCaM7, Ca(2+) and ROS are three important regulators involved in early fiber elongation. GhCaM7 might modulate ROS production and act as a molecular link between Ca(2+) and ROS signal pathways in early fiber development.

Keywords: Gossypium hirsutum (cotton); calcium signaling; calmodulin; feedback regulation; fiber elongation; reactive oxygen species (ROS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Calcium / metabolism*
  • Calmodulin / genetics
  • Calmodulin / metabolism*
  • Cotton Fiber
  • Genes, Plant*
  • Gossypium / genetics
  • Gossypium / growth & development
  • Gossypium / metabolism*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Reactive Oxygen Species / metabolism*
  • Seeds / growth & development
  • Seeds / metabolism*
  • Signal Transduction

Substances

  • Arabidopsis Proteins
  • CaM7 protein, Arabidopsis
  • Calmodulin
  • Plant Proteins
  • Reactive Oxygen Species
  • Calcium