Are superoxide dismutase 2 and nitric oxide synthase polymorphisms associated with idiopathic infertility?

Antioxid Redox Signal. 2014 Aug 1;21(4):565-9. doi: 10.1089/ars.2014.5831. Epub 2014 Feb 25.


The aim of this study was to investigate in a case-control study the associations between idiopathic infertility and antioxidant gene polymorphisms. One hundred ten infertile subjects (58 women and 52 men) with a history of idiopathic infertility and 69 fertile subjects (35 women and 34 men) with no history of infertility were included by three hospital departments of reproductive biology in the NCT01093378 French government clinical trial. Genotyping was assessed by real-time polymerase chain reaction with TaqMan assay. We examined genetic polymorphisms affecting five antioxidant enzymes: manganese superoxide dismutase (MnSOD), myeloperoxidase (MPO), glutathione peroxidase 1 (GPx1), catalase (CAT), and endothelial nitric oxide synthase (eNOS). The presence of at least 1 Ala-MnSOD allele (rs4880) increased significantly the risk of infertility (odds ratio [OR] 2.94; 95% confidence interval [CI], 1.14, 7.60; p=0.03) in male subjects. Moreover, the presence of 2 G-eNOS allele (rs1799983) increased significantly the risk of infertility in both men and women (OR 1.91; 95% CI, 1.04, 3.54; p=0.04). Our observations lead to the hypothesis that the genetic susceptibility modulating oxidative stress may represent a risk factor for male idiopathic infertility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Catalase / genetics
  • Female
  • Genetic Predisposition to Disease
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase GPX1
  • Humans
  • Infertility / genetics*
  • Male
  • Nitric Oxide Synthase / genetics*
  • Nitric Oxide Synthase Type III / genetics
  • Odds Ratio
  • Polymorphism, Genetic*
  • Polymorphism, Single Nucleotide
  • Risk Factors
  • Superoxide Dismutase / genetics*


  • Catalase
  • Glutathione Peroxidase
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type III
  • Superoxide Dismutase
  • superoxide dismutase 2
  • Glutathione Peroxidase GPX1
  • GPX1 protein, human

Associated data