Royal jelly attenuates azathioprine induced toxicity in rats

Environ Toxicol Pharmacol. 2014 Jan;37(1):431-7. doi: 10.1016/j.etap.2013.12.010. Epub 2013 Dec 31.

Abstract

In the present study, we investigated the potential protective effects of royal jelly against azathioprine-induced toxicity in rat. Intraperitoneal administration of azathioprine (50 mg/kgB.W.) induced a significant decrease in RBCs count, Hb concentration, PCV%, WBCs count, differential count and platelet count, hepatic antioxidant enzymes (reduced glutathione and glutathione s-transferase) and increase of serum transaminases (alanine aminotransferase and aspartate aminotransferase enzymes) activities, alkaline phosphatase and malondialdehyde formation. Azathioprine induced hepatotoxicity was reflected by marked pathological changes in the liver. Oral administration of royal jelly (200 mg/kgB.W.) was efficient in counteracting azathioprine toxicity whereas it altered the anemic condition, leucopenia and thrombocytopenia induced by azathioprine. Furthermore, royal jelly exerted significant protection against liver damage induced by azathioprine through reduction of the elevated activities of serum hepatic enzymes. Moreover, royal jelly blocked azathioprine-induced lipid peroxidation through decreasing the malondialdehyde formation. In conclusion, royal jelly possesses a capability to attenuate azathioprine-induced toxicity.

Keywords: Azathioprine; Hepatotoxicity; Oxidative stress; Royal jelly.

MeSH terms

  • Alanine Transaminase / blood
  • Alkaline Phosphatase / blood
  • Anemia / chemically induced
  • Anemia / drug therapy
  • Anemia / metabolism
  • Anemia / pathology
  • Animals
  • Antimetabolites, Antineoplastic / toxicity*
  • Aspartate Aminotransferases / blood
  • Azathioprine / toxicity*
  • Chemical and Drug Induced Liver Injury / drug therapy*
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / metabolism
  • Chemical and Drug Induced Liver Injury / pathology
  • Fatty Acids / pharmacology
  • Fatty Acids / therapeutic use*
  • Glutathione / metabolism
  • Leukopenia / chemically induced
  • Leukopenia / drug therapy
  • Leukopenia / metabolism
  • Leukopenia / pathology
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Protective Agents / pharmacology
  • Protective Agents / therapeutic use*
  • Rats
  • Rats, Wistar
  • Thrombocytopenia / chemically induced
  • Thrombocytopenia / drug therapy
  • Thrombocytopenia / metabolism
  • Thrombocytopenia / pathology

Substances

  • Antimetabolites, Antineoplastic
  • Fatty Acids
  • Protective Agents
  • Aspartate Aminotransferases
  • Alanine Transaminase
  • Alkaline Phosphatase
  • Glutathione
  • royal jelly
  • Azathioprine