Molecular Characterization of Mycoplasma Genitalium Species-Specific and Cross-Reactive Determinants: Identification of an Immunodominant Protein of M. Genitalium

Isr J Med Sci. 1987 May;23(5):453-7.

Abstract

Species-specific proteins of Mycoplasma genitalium as well as proteins cross-reactive with M. pneumoniae have been identified using monoclonal antibodies generated against these mycoplasmas in enzyme-linked immunosorbent assays (ELISA) and Western blot analyses. Specificity of the antibodies was examined using M. hominis, M. orale M. salivarium and Acholeplasma laidlawii. A 140-kDa (kilodalton) protein of M. genitalium that appeared to be immunodominant in mice was also shown by radioimmunoprecipitation to be immunodominant in experimentally infected chimpanzees.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / immunology
  • Antibodies, Monoclonal / immunology
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins / immunology*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Female
  • Immunoelectrophoresis
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mycoplasma / classification
  • Mycoplasma / immunology*
  • Pan troglodytes
  • Species Specificity

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Proteins
  • Epitopes