Crystal structure determination of anti-DNA Fab A52

Proteins. 2014 Aug;82(8):1674-8. doi: 10.1002/prot.24514. Epub 2014 Feb 18.


A52 is a murine monoclonal antibody isolated from autoimmune New Zealand Black/New Zealand White F1 mice that recognizes single and double stranded DNA. This mouse strain spontaneously develops systemic lupus erythematosus-like symptoms and has served as a model for that disease for many years. The 1.62 Å crystal structure of the A52 Fab fragment reveals an H3 complementarity determining region with four closely spaced arginine residues, creating a positively charged surface to accommodate bound DNA.

Keywords: DNA; Fab; antibody; lupus erythematosus; systemic.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Antinuclear / chemistry*
  • Antibodies, Antinuclear / immunology*
  • Complementarity Determining Regions / chemistry
  • Complementarity Determining Regions / immunology
  • DNA / immunology*
  • Immunoglobulin Fab Fragments / chemistry*
  • Immunoglobulin Fab Fragments / immunology*
  • Lupus Erythematosus, Systemic / immunology
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Sequence Alignment


  • Antibodies, Antinuclear
  • Complementarity Determining Regions
  • Immunoglobulin Fab Fragments
  • DNA