Increased collagen III in culled chicken meat after feeding dietary wood charcoal and vinegar contributes to palatability and tenderness

Anim Sci J. 2014 Apr;85(4):468-80. doi: 10.1111/asj.12160. Epub 2014 Jan 23.


We comprehensively evaluated meat quality in chickens fed a diet consisting of wood charcoal and vinegar (WCV) using food scientific and histological approaches. In culled hens, lipid and fatty acid in Musculus semimembranosus, cooking loss and sensory tests of whole thigh meat, and meat texture of breast meat were observed. In male broilers, cross section of M. semimembranosus was used for observations on muscle area, perimysium, non-collagen total protein and total collagen content, and anti-collagen I and III reactions. In frozen male broilers, conventional morphology of M. semimembranosus as well as chicken anti-collagen III reaction to selected muscles of thigh meat and breast meat were compared between the control and WCV-fed birds. Increased lipid and fatty acids, decreased cooking loss, high score in total evaluation for sensory test of thigh meat, and decreased meat texture values were observed for culled hens fed WCV. The higher values of muscle area, total collagen and collagen III were observed for broilers fed WCV. No perimysium collapse for M. semitendinosus or increased collagen III reactions of M. tensor fasciae latae, the flexor muscle group and M. pectoralis superficialis were observed for frozen muscles in the WCV group. These total results suggest that WCV produces palatable and tender meat by increasing collagen III.

Keywords: chicken; collagen; meat; wood charcoal; wood vinegar.

MeSH terms

  • Acetic Acid / administration & dosage*
  • Acetic Acid / pharmacology*
  • Animal Feed*
  • Animal Nutritional Physiological Phenomena / physiology*
  • Animals
  • Charcoal / administration & dosage*
  • Charcoal / pharmacology*
  • Chickens / metabolism*
  • Collagen Type III / analysis*
  • Collagen Type III / metabolism*
  • Dietary Supplements*
  • Fatty Acids / metabolism
  • Female
  • Lipid Metabolism / drug effects
  • Male
  • Meat / analysis*
  • Muscle, Skeletal / metabolism


  • Collagen Type III
  • Fatty Acids
  • Charcoal
  • Acetic Acid