Antioxidant characterization of oak extracts combining spectrophotometric assays and chemometrics

ScientificWorldJournal. 2013 Dec 25:2013:134656. doi: 10.1155/2013/134656. eCollection 2013.


Antioxidant characteristics of leaves, twigs, and acorns from two Serbian oak species Quercus robur L. and Quercus petraea L. from Vojvodina province (northern Serbia) were investigated. 80% ethanol (in water) extracts were used for antiradical power (ARP) determinations against DPPH(•), (•)NO, and O2 (•-) radicals, ferric reducing antioxidant power (FRAP), total phenol, tannin, flavonoid, and proanthocyanidin contents. Permanganate reducing antioxidant capacity (PRAC) was determined using water extracts. Beside, mentioned parameters, soluble proteins, lipid peroxidation (LP), pigments and proline contents were also determined. The data of different procedures were compared and analyzed by multivariate techniques (correlation matrix calculation and principal component analysis (PCA)). PCA found that investigated organs of two different oak tree species possess similar antioxidant characteristics. The superior antioxidant characteristics showed oak leaves over twigs and acorns and seem to be promising source of antioxidants with possible use in industry and pharmacy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / chemistry*
  • Chemistry Techniques, Analytical
  • Ethanol / chemistry
  • Flavonoids / chemistry
  • Free Radicals / chemistry
  • Hydroxyl Radical / chemistry
  • Lipid Peroxidation
  • Multivariate Analysis
  • Phenol / chemistry
  • Pigmentation
  • Plant Extracts / chemistry*
  • Principal Component Analysis
  • Proanthocyanidins / chemistry
  • Proline / chemistry
  • Quercus / chemistry*
  • Spectrophotometry*
  • Tannins / chemistry
  • Trees


  • Antioxidants
  • Flavonoids
  • Free Radicals
  • Plant Extracts
  • Proanthocyanidins
  • Tannins
  • proanthocyanidin
  • Hydroxyl Radical
  • Phenol
  • Ethanol
  • Proline