High-efficiency solid phase peptide synthesis (HE-SPPS)

Org Lett. 2014 Feb 7;16(3):940-3. doi: 10.1021/ol4036825. Epub 2014 Jan 23.

Abstract

A series of improvements to the standard solid phase peptide synthesis (SPPS) process allowing for significant gains in product purity along with only a 4 min standard cycle time and a 90% reduction in total waste produced is reported. For example, syntheses of the well-known (65-74)acyl carrier protein (ACP) and (1-42)β-amyloid peptides were accomplished with 93 and 72% purity (UPLC-MS) in only 44 and 229 min, respectively.

MeSH terms

  • Acyl Carrier Protein / chemical synthesis*
  • Acyl Carrier Protein / chemistry
  • Amyloid beta-Peptides / chemical synthesis*
  • Amyloid beta-Peptides / chemistry
  • Molecular Structure
  • Oligopeptides / chemical synthesis*
  • Oligopeptides / chemistry
  • Peptide Fragments / chemical synthesis*
  • Peptide Fragments / chemistry
  • Solid-Phase Synthesis Techniques

Substances

  • Acyl Carrier Protein
  • Amyloid beta-Peptides
  • Oligopeptides
  • Peptide Fragments