The primary structure of human chromogranin A and pancreastatin

J Biol Chem. 1987 Dec 15;262(35):17026-30.


A full-length clone encoding human chromogranin A has been isolated from a lambda gt10 cDNA library of a human pheochromocytoma. The nucleotide sequence reveals that human chromogranin A is a 439-residue protein preceded by an 18-residue signal peptide. Comparison of the protein sequence of human chromogranin A with that of bovine chromogranin A shows high conservation of the NH2-terminal and COOH-terminal domains as well as the potential dibasic cleavage sites, whereas the middle portion shows remarkable sequence variation (36%). This part of human chromogranin A contains a sequence homologous to porcine pancreastatin at residues 250-301. The sequence variation in this part of human chromogranin A compared to porcine pancreastatin is 32% and thus of the same magnitude as that between human and bovine chromogranin A. Therefore, the difference between porcine pancreastatin and the corresponding portions of bovine or human chromogranin A can be explained by species variation, suggesting that pancreastatin is derived from chromogranin A itself rather than a protein that is only similar to chromogranin A. Moreover, the pancreastatin sequence contained in human chromogranin A is flanked by sites for proteolytic processing. Together, these observations suggest that human chromogranin A may be the precursor for a human pancreastatin molecule and possibly for other, as yet unidentified, biologically active peptides.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Chromogranin A
  • Chromogranins / analysis*
  • Chromogranins / genetics
  • Molecular Sequence Data
  • Nerve Tissue Proteins / analysis*
  • Pancreatic Hormones / analysis*
  • Pancreatic Hormones / genetics
  • Poly A / analysis
  • RNA / analysis
  • RNA, Messenger
  • Swine


  • CHGA protein, human
  • Chromogranin A
  • Chromogranins
  • Nerve Tissue Proteins
  • Pancreatic Hormones
  • RNA, Messenger
  • pancreastatin
  • Poly A
  • RNA

Associated data

  • GENBANK/J03483