Detection of flavivirus antibodies in human serum by epitope-blocking immunoassay

J Med Virol. 1987 Oct;23(2):165-73. doi: 10.1002/jmv.1890230209.

Abstract

Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (greater than 6 months after illness) convalescent sera were tested, the epitope-blocking immunoassays were superior to the hemagglutination inhibition test and comparable to the plaque reduction neutralization for identifying subjects immune to dengue, to Japanese encephalitis, or both viruses.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / analysis*
  • Antigens, Viral / immunology*
  • Binding, Competitive
  • Cross Reactions
  • Dengue / immunology
  • Dengue Virus / immunology*
  • Encephalitis Virus, Japanese / immunology*
  • Encephalitis, Japanese / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Flavivirus / immunology*
  • Hemagglutination Inhibition Tests
  • Humans
  • Longitudinal Studies
  • Neutralization Tests
  • Predictive Value of Tests

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Epitopes