[Inhibitory effect of microwave radiation on proliferation of human pancreatic cancer JF305 cells and its mechanism]

Wei Sheng Yan Jiu. 2013 Nov;42(6):1008-11.
[Article in Chinese]


Objective: To investigate on the proliferation effect of different intensities 2450 MHz microwave radiation on human pancreatic cancer JF305 cells and its possible mechanism.

Methods: JF305 cells were radiated by intensity of 2.5, 5.0, 10.0, 15.0 and 20.0 mW/cm2 microwave for 20 min. The proliferation capacity of JF305 was measured by MTT assays, Annexin V-FITC and PI staining was used for detecting cell apoptosis. The activity of Caspase-3 was examined. The expressions of Caspase-3 and HSP 70 protein after the cell treatment with microwave were detected by Western blotting.

Results: After microwave radiation, the proliferation inhibition rates of JF305 cells were significantly higher compared with control group. Annexin V-FITC and PI staining result showed that microwave radiation could induce cell apoptosis. Caspase-3 increased after radiated by microwave, compared with control group (P < 0.05). Results of Western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group.

Conclusion: Microwave radiation can inhibit the proliferation of JF305 cells, the possible mechanism may be related with inducing cell apoptosis by changing of stress level.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / radiation effects*
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • Cell Proliferation / radiation effects*
  • HSP70 Heat-Shock Proteins / metabolism
  • Humans
  • Microwaves*
  • Pancreatic Neoplasms / pathology*


  • HSP70 Heat-Shock Proteins
  • CASP3 protein, human
  • Caspase 3