A novel epitope of the LFA-1 antigen which can distinguish killer effector and suppressor cells in human CD8 cells

Nature. 1987 Dec 3-9;330(6147):479-82. doi: 10.1038/330479a0.

Abstract

The CD4 subset of cells displays helper/inducer activity and recognizes class II antigens of the major histocompatibility complex (MHC), while the CD8 subset recognizes class I MHC antigens and exhibits cytotoxic or suppressor function. Considerable functional as well as corresponding phenotypic heterogeneity exists within the two major T cell subsets. Although the CD8+ population contains pre-cytotoxic, cytotoxic, pre-suppressor and suppressor effector T cells, these distinctions still rest largely on the use of functional assays. Attempts have been made to define the CD8+ precursor of the killer cell with new monoclonal antibodies. But more precise phenotypic distinctions between the functional subpopulations within CD8+ cells will be needed. We have now developed a monoclonal antibody, anti-S6F1 which can distinguish killer effector and suppressor effector cells in CD8 lymphocyte populations. The cell-surface structure defined by this antibody comprises two glycoproteins with relative molecular mass (Mr) 180K and 95K respectively. Also sequential immunoprecipitation studies and two dimensional gel electrophoresis indicate that anti-S6F1 recognizes a novel epitope on the LFA-1 antigen.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte / immunology*
  • Antigens, Surface / analysis*
  • Antigens, Surface / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Epitopes / analysis*
  • Humans
  • Immunosorbent Techniques
  • Killer Cells, Natural / immunology*
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Function-Associated Antigen-1
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • T-Lymphocytes, Cytotoxic / immunology
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Epitopes
  • Lymphocyte Function-Associated Antigen-1