Flow cytometry applications for the analysis of chemokine receptor expression and function

Cytometry A. 2014 Apr;85(4):292-301. doi: 10.1002/cyto.a.22439. Epub 2014 Jan 24.


Chemokine receptors play an important role in leukocyte migration, both in physiological and pathological conditions, and the interest in new methodologies for their detection is increasing. In this review, we focused on chemokine receptors detection through flow cytometric approaches, including the use of specific antibodies and fluorescent chemokines, and on approaches aimed at the analysis of their functions, from intracellular trafficking to signaling activities.

Keywords: antibodies; chemokine receptor; flow cytometry; fluorophores.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Flow Cytometry / methods*
  • Humans
  • Leukocytes / metabolism
  • Receptors, Chemokine / analysis*


  • Receptors, Chemokine