Cross-linking of streptomycin to the 16S ribosomal RNA of Escherichia coli

Biochemistry. 1987 Sep 22;26(19):6227-32. doi: 10.1021/bi00393a041.


[3H]Dihydrostreptomycin was cross-linked to the 30S ribosomal subunit from Escherichia coli with the bifunctional reagent nitrogen mustard. The cross-linking primarily involved the 16S RNA. To localize the site of cross-linking of streptomycin to the 16S RNA, we hybridized RNA labeled with streptomycin to restriction fragments of the 16S RNA gene. Labeled RNA hybridized to DNA fragments corresponding to bases 892-917 and bases 1394-1415. These two segments of the ribosomal RNA must be juxtaposed in the ribosome, since there is a single binding site for streptomycin. This region has been implicated both in the decoding site and in the binding of initiation factor IF-3, indicating its functional importance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents*
  • Dihydrostreptomycin Sulfate / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Mechlorethamine / metabolism*
  • Models, Molecular
  • Nucleic Acid Conformation
  • Plasmids
  • RNA, Ribosomal / metabolism*
  • RNA, Ribosomal, 16S / metabolism*
  • Ribosomes / metabolism


  • Cross-Linking Reagents
  • RNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Mechlorethamine
  • Dihydrostreptomycin Sulfate