Coagulation factor and hemostatic protein content of canine plasma after storage of whole blood at ambient temperature

J Vet Intern Med. 2014 Mar-Apr;28(2):571-5. doi: 10.1111/jvim.12277. Epub 2014 Jan 27.


Background: Standard practice in canine blood banking is to produce fresh frozen plasma (FFP) by separating and freezing plasma produced from blood within 8 hours of collection. Within canine blood donation programs, this can limit the number of units collected.

Hypothesis/objectives: The aim was to compare the coagulation factor and hemostatic protein content (CF&HPC) of plasma produced from blood stored at ambient temperature for 8, 12, and 24 hours. Another aim was to compare the CF&HPC between Greyhound types and other breeds.

Animals: None.

Methods: In vitro study. A convenience sample of 58 units of canine blood from a blood donor pool was processed to prepare and freeze plasma 8, 12, or 24 hours following collection.

Results: Regardless of time of processing, the units contained therapeutic CF&HPC. Frozen plasma prepared after 24 hours had significantly higher factor VIII (P = .014) and factor X (P = .03) when compared with the frozen plasma prepared at 8 hours. Factor X (P < .01), fibrinogen (P < .01), and vWF (P = .04) were significantly lower in plasma collected from Greyhound types than in plasma collected from other breeds.

Conclusions and clinical importance: Storing whole blood for up to 24 hours is a suitable method for producing FFP. Lower values for some coagulation factors and hemostatic proteins in plasma produced from Greyhound types would not preclude these dogs as FFP donors.

Keywords: Factor VIII; Hemostasis; Transfusion; von Willebrand factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Coagulation Factors / analysis*
  • Blood Specimen Collection / veterinary
  • Dogs / blood*
  • Factor IX / analysis
  • Factor V / analysis
  • Factor VII / analysis
  • Factor VIII / analysis
  • Factor X / analysis
  • Female
  • Fibrinogen / analysis
  • Male
  • Prothrombin / analysis
  • Species Specificity
  • von Willebrand Factor / analysis


  • Blood Coagulation Factors
  • von Willebrand Factor
  • Factor V
  • Factor VII
  • Prothrombin
  • Factor VIII
  • Factor IX
  • Factor X
  • Fibrinogen