Regulation of goby intestinal ion absorption by the calcium messenger system

J Exp Zool. 1987 Oct;244(1):67-78. doi: 10.1002/jez.1402440109.

Abstract

The role of the calcium messenger system in the regulation of ion absorption across the teleost intestine was studied using pharmacological intervention. Radiochloride transport was independent of external Ca2+ over the range 10 microM to 2.5 mM. Treatment with the Ca2+ ionophore A23187 (to hyperpolarization of the apical membrane potential of intestinal epithelial cells. The Ca2+-calmodulin antagonists trifluoperazine (TFP) and calmidazolium (R24571) produced opposite effects, i.e., stimulation of Cl- absorption and cellular depolarization. Treatment with TFP or R24571 will block or override the inhibitory action of A23187. These data suggest a regulatory role for Ca2+ in the control of intestinal NaCl absorption and mediation via calmodulin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Animals
  • Calcimycin / pharmacology
  • Calcium / pharmacology
  • Calcium / physiology*
  • Calmodulin / antagonists & inhibitors
  • Female
  • Fishes / physiology*
  • Imidazoles / pharmacology
  • Intestinal Absorption* / drug effects
  • Ion Channels / metabolism*
  • Male
  • Microscopy, Electron
  • Trifluoperazine / pharmacology

Substances

  • Calmodulin
  • Imidazoles
  • Ion Channels
  • Trifluoperazine
  • Calcimycin
  • calmidazolium
  • Calcium
  • 1-Methyl-3-isobutylxanthine