Methylation-sensitive amplified polymorphism (MSAP) marker to investigate drought-stress response in Montepulciano and Sangiovese grape cultivars

Methods Mol Biol. 2014:1112:151-64. doi: 10.1007/978-1-62703-773-0_10.

Abstract

Methylation-sensitive amplified polymorphism (MSAP) is a technique developed for assessing the extent and pattern of cytosine methylation and has been applied to genomes of several species (Arabidopsis, grape, maize, tomato, and pepper). The technique relies on the use of isoschizomers that differ in their sensitivity to methylation.

MeSH terms

  • DNA Methylation*
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Droughts*
  • Electrophoresis, Polyacrylamide Gel
  • Genetic Markers / genetics
  • Nucleic Acid Amplification Techniques / methods*
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA
  • Silver Staining
  • Species Specificity
  • Stress, Physiological / genetics*
  • Vitis / genetics*
  • Vitis / physiology*

Substances

  • DNA, Plant
  • Genetic Markers