Detecting histone modifications in plants

Methods Mol Biol. 2014;1112:165-75. doi: 10.1007/978-1-62703-773-0_11.

Abstract

Histone modifications play an essential role in chromatin-associated processes including gene regulation and epigenetic inheritance. It is therefore very important to quantitatively analyze histone modifications at both the single gene and whole genome level. Here, we describe a robust chromatin immunoprecipitation (ChIP) method for Arabidopsis, which could be adapted for other plant species. This method is compatible with multiple downstream applications including qPCR, tilling arrays, and high-throughput sequencing.

MeSH terms

  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Chromatin / genetics
  • Chromatin Immunoprecipitation / methods*
  • DNA Fragmentation
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • Formaldehyde / pharmacology
  • Histones / metabolism*
  • Protein Processing, Post-Translational*

Substances

  • Chromatin
  • DNA, Plant
  • Histones
  • Formaldehyde