[Carbohydrate restriction in the larval diet causes oxidative stress in adult insects of Drosophila melanogaster]

Ukr Biokhim Zh (1999). 2013 Sep-Oct;85(5):61-72. doi: 10.15407/ubj85.05.061.
[Article in Ukrainian]

Abstract

The influence of 20 and 1% glucose and fructose, which were components of larval diet, on the level of oxidized proteins and lipids, low molecular mass antioxidant content as well as activities of antioxidant and associated enzymes in adult fruit fly Drosophila melanogaster were investigated. The restriction of carbohydrates in larval diet leads to oxidative stress in adult insects. It is supported by 40-50% increased content of protein carbonyl groups and by 60-70% decreased level of protein thiol groups as well as by a 4-fold increase of lipid peroxide content in 2-day-old flies of both sexes, developed on the diet with 1% carbohydrates. Oxidative stress, induced by carbohydrate restriction of the larval diet, caused the activation of antioxidant defence, differently exhibited in male and female fruit flies. Caloric restriction increased activity of superoxide dismutase and thioredoxin reductase associating only in males with 2-fold higher activity of NADPH-producing enzymes--glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase. Carbohydrate restriction in the larval diet caused the increase of uric acid content, but the decrease in catalase activity in males. In females the values of these parameters were changed in opposite direction compared with males. The obtained results let us conclude the different involvement of low molecular mass antioxidants, glutathione and uric acid, and antioxidant enzyme catalase in the protection of male and female fruit fly macromolecules against oxidative damages, caused by calorie restriction of larval diet.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbohydrates / deficiency*
  • Catalase / metabolism
  • Dietary Carbohydrates / administration & dosage*
  • Dietary Carbohydrates / metabolism
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / metabolism*
  • Female
  • Fructose / administration & dosage
  • Fructose / metabolism
  • Glucose / administration & dosage
  • Glucose / metabolism
  • Glucosephosphate Dehydrogenase / metabolism
  • Glutathione / metabolism
  • Insect Proteins / metabolism
  • Isocitrate Dehydrogenase / metabolism
  • Larva / growth & development
  • Larva / metabolism
  • Lipid Metabolism
  • Lipid Peroxidation
  • Male
  • Oxidative Stress*
  • Protein Carbonylation
  • Sex Factors
  • Superoxide Dismutase / metabolism
  • Thioredoxin-Disulfide Reductase / metabolism
  • Time Factors
  • Uric Acid / metabolism

Substances

  • Carbohydrates
  • Dietary Carbohydrates
  • Insect Proteins
  • Uric Acid
  • Fructose
  • Isocitrate Dehydrogenase
  • Glucosephosphate Dehydrogenase
  • Catalase
  • Superoxide Dismutase
  • Thioredoxin-Disulfide Reductase
  • Glutathione
  • Glucose