Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants

Biochimie. 1987 Aug;69(8):885-9. doi: 10.1016/0300-9084(87)90216-1.


Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of E. coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Drug Resistance, Microbial
  • Escherichia coli / genetics
  • Escherichia coli / metabolism*
  • Microbial Sensitivity Tests
  • Mutation
  • Plasmids
  • RNA, Bacterial / analysis*
  • RNA, Ribosomal / analysis*
  • RNA, Ribosomal, 16S / analysis*
  • Spectinomycin / pharmacology*


  • RNA, Bacterial
  • RNA, Ribosomal
  • RNA, Ribosomal, 16S
  • Spectinomycin