Influence of probe structure on unique (regiospecific) cleavage of RNA by RNase H

V G Metelev; G V Zayakina; I L Ryabushenko; N F Krynetskaya; E A Romanova; T S Oretskaya; Z A Shabarova

doi:10.1016/0014-5793(88)81429-7

Influence of probe structure on unique (regiospecific) cleavage of RNA by RNase H

FEBS Lett. 1988 Jan 4;226(2):232-4. doi: 10.1016/0014-5793(88)81429-7.

Authors

V G Metelev¹, G V Zayakina, I L Ryabushenko, N F Krynetskaya, E A Romanova, T S Oretskaya, Z A Shabarova

Affiliation

¹ Chemistry Department, Moscow State University, USSR.

PMID: 2448163
DOI: 10.1016/0014-5793(88)81429-7

Abstract

Chimeric oligo(ribo-deoxyribo)nucleotides with an internucleotide pyrophosphate bond are novel probes for regiospecific hydrolysis of RNA by RNase H. It has been shown that the use of d(TGTGTAT)ppGCCAU leads to unique hydrolysis of the TMV RNA fragment pAAUGGCAUACAC between C10 and A11.

MeSH terms

Base Sequence
Endoribonucleases / metabolism*
Escherichia coli / enzymology
Oligodeoxyribonucleotides
Oligoribonucleotides
RNA*
Ribonuclease H
Substrate Specificity

Substances

Oligodeoxyribonucleotides
Oligoribonucleotides
RNA
Endoribonucleases
Ribonuclease H