Two solid-phase assays have been used to measure the affinity of IgG1 and IgG4 antibodies to tetanus toxoid (TT) in whole sera. In the first assay, low affinity antibodies were selectively inhibited from binding to solid-phase antigen by a mild chaotropic agent and in the second assay, high affinity antibodies were preferentially inhibited from binding by low concentrations of free antigen. Results from both assays suggest that IgG4 antibodies to TT are of lower functional affinity than IgG1 antibodies and that, in addition, IgG4 responses to TT display a more restricted affinity heterogeneity.