Purification and reconstitution of the calcium release channel from skeletal muscle

Nature. 1988 Jan 28;331(6154):315-9. doi: 10.1038/331315a0.


The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca2+-release channels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Ion Channels / analysis*
  • Ion Channels / metabolism
  • Ion Channels / ultrastructure
  • Liposomes
  • Membrane Potentials
  • Rabbits
  • Receptors, Cholinergic / isolation & purification*
  • Receptors, Cholinergic / metabolism
  • Ryanodine Receptor Calcium Release Channel
  • Sarcoplasmic Reticulum / analysis*
  • Sarcoplasmic Reticulum / ultrastructure


  • Ion Channels
  • Liposomes
  • Receptors, Cholinergic
  • Ryanodine Receptor Calcium Release Channel
  • Calcium