Serological diagnosis with recombinant N antigen for hantavirus infection

Virus Res. 2014 Jul 17:187:77-83. doi: 10.1016/j.virusres.2013.12.040. Epub 2014 Jan 31.

Abstract

Hantaviruses are causative agents of two rodent-borne zoonoses, hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. Serological examinations to detect hantavirus antibodies have been most widely used for surveillance among humans and rodent reservoirs. Here, we will review antigenic structure of nucleocapsid (N) protein of hantaviruses and application of recombinant N protein as diagnostic antigen for screening and serotyping.

Keywords: Alternative neutralization test; Immunochoromatography.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Antigens, Viral / blood*
  • Capsid Proteins / chemistry*
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology
  • Chromatography, Affinity
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Hantavirus Infections / blood
  • Hantavirus Infections / diagnosis*
  • Hantavirus Infections / immunology
  • Hantavirus Infections / virology
  • Humans
  • Neutralization Tests
  • Orthohantavirus / classification
  • Orthohantavirus / genetics
  • Orthohantavirus / immunology*
  • Phylogeny
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Viral Core Proteins / chemistry*
  • Viral Core Proteins / genetics
  • Viral Core Proteins / immunology

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins
  • Recombinant Proteins
  • Viral Core Proteins
  • nucleocapsid protein, Hantaan virus